Part:BBa_J72169

O7 biosynthesis cluster

Encodes the O7 biosynthesis cluster cloned from strain ATCC 23503. It is sufficient to confer an O7 phenotype to MG1655 (but not MC1061), including resistance to bacteriophage T7 and horse serum. Referred to internally as jtk3113.

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This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 11729
  Illegal SpeI site found at 7288
  Illegal SpeI site found at 11619
  Illegal PstI site found at 1537
  Illegal PstI site found at 1685
  Illegal PstI site found at 11323
  Illegal PstI site found at 12978
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 6302
  Illegal SpeI site found at 7288
  Illegal SpeI site found at 11619
  Illegal PstI site found at 1537
  Illegal PstI site found at 1685
  Illegal PstI site found at 11323
  Illegal PstI site found at 12978
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 11729
  Illegal SpeI site found at 7288
  Illegal SpeI site found at 11619
  Illegal PstI site found at 1537
  Illegal PstI site found at 1685
  Illegal PstI site found at 11323
  Illegal PstI site found at 12978
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 11729
  Illegal SpeI site found at 7288
  Illegal SpeI site found at 11619
  Illegal PstI site found at 1537
  Illegal PstI site found at 1685
  Illegal PstI site found at 11323
  Illegal PstI site found at 12978
  Illegal AgeI site found at 1822
  Illegal AgeI site found at 13043
  Illegal AgeI site found at 13711
  Illegal AgeI site found at 14398
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 3179